A novel UV-Spectroscopy and RP-HPLC method has been developed for the rosuvastatin calcium (ROSU) and clopidogrel bisulphate (CLOP) in tablet dosage form and validated according to ICH guidelines. The method is a simple, accurate, specific, precise, reproducible and sensitive. The Prontosil C18 column of dimension 250 x 4.6 mm i.d., particle size 10 m utilizing the system methanol: water (80:20 ratio) the mobile phase at the rate of 1 ml/min by employing 240 nm detector, where the drug retention time at 3.483 min of rosuvastatin calcium and 4.983 min of clopidogrel bisulphate. The method was observed to be linear (r2 = 0.999) over the range of 50-150 g/ml. The method was observed λmax at 240 nm. Employing the four different concentration of mobile phase and finally selected, since it gives sharp reproducible retention time. Employed the sample 20 µl, %RSD of ROSU 1.017 and CLOP 0.173, theoretical plates ROSU 7797.53 and CLOP 8257.53, tailing factor ROSU 1.1787 and CLOP 1.074, limits 2 NMT, accuracy ROSU 0.37 %RSD, recovery 99.59% and CLOP 0.18 %RSD, recovery of 100.41%. The method showed the good efficacy and results, no interference from the diluents or excipients was encountered. The method indicated the future scope in quality control, the estimation of rosuvastatin calcium and clopidogrel bisulphate for routine quality analysis investigation.
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